Identification of Qtls for Brown Planthopper (nilaparvata Lugens Stal.) Resistance in Ril Mapping Population of Rice (oryza Sativa L.)

The brown planthopper (Nilaparvata lugens Stal.) is one of the major insect pests of rice (Oryza sativa L.). Exploitation of Host Plant Resistance is a major component to manage this pest. The development of rice varieties that are resistant to the brown planthopper is an important objective in current breeding programmes. This study was undertaken to identify QTLs for BPH resistance from an elite local accession. In the present study, two hundred seventy lines of recombinant inbred lines (RILs) of Danteshwari/Dagad Deshi were evaluated under glass house condition for brown planthopper resistance. Two leaf stage seedlings were artificially infested in the screen house with second and third instar brown planthopper nymphs, using standard seed box technique. Reactions of the seedlings were recorded seven to ten days after infestation when the susceptible check TN1 was completely killed. The lines found resistant for BPH were further tested by probing mark test and feeding test. The genotypic data of the whole population was developed using SSR and SNP markers. The genotypic data thus obtained was used for QTL analysis using single marker analysis to find out association between markers and trait under study. 10 SSR and 3 SNP markers showed significant association with the trait.